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Cell pausing method for adipose tissue derived mesenchymal stem cells: comparison of Petaka G3 and ordinary flask

  
@article{SCI33854,
	author = {Jeanne Adiwinata Pawitan and Isabella Kurnia Liem and Tera Dria Kispa and Evah Luviah and Fajar Mujadid and Novialdi Novialdi},
	title = {Cell pausing method for adipose tissue derived mesenchymal stem cells: comparison of Petaka G3 and ordinary flask},
	journal = {Stem Cell Investigation},
	volume = {7},
	number = {0},
	year = {2019},
	keywords = {},
	abstract = {Background: Various methods of cell pausing were applied to prevent proliferation of cultured cells, which either required special medium, using complicated encapsulation method, or using a special flask (Petaka G3 flask). Therefore, this study aimed to develop an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT-MSCs) using ordinary basal medium and flask that yielded AT-MSCs, which were compliant with the requirements of International society for cell therapy (ISCT).
Methods: We assessed cell numbers and viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs, which were cultured until 80% confluent and then placed at 4 °C or room temperature for three to seven days. We assessed those parameters between fully filled and sealed ordinary flasks compared to Petaka G3 flasks.
Results: Cell numbers, viabilities, and percentages of CD73, CD90, CD105, and lineage negative, as well as differentiation potentials of AT-MSCs at room temperature were better than at 4 °C, and at room temperature, results of ordinary flasks were comparable to Petaka G3 flasks.
Conclusion: We have developed an easy and economical method of cell pausing method for adipose tissue derived mesenchymal stem cells (AT- MSCs), using ordinary basal medium and flask.},
	issn = {2306-9759},	url = {http://sci.amegroups.com/article/view/33854}
}