Current development of chimeric antigen receptor T-cell therapy
Review Article

Current development of chimeric antigen receptor T-cell therapy

Jiasheng Wang1, Yongxian Hu2,3,4, He Huang2,3,4

1Department of Internal Medicine, MetroHealth Medical Center, Case Western Reserve University, Cleveland, OH, USA; 2Bone Marrow Transplantation Center, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China; 3Institute of Hematology, Zhejiang University, Hangzhou 310058, China; 4Zhejiang Province Engineering Laboratory for Stem Cell and Immunity Therapy, Hangzhou 310058, China

Contributions: (I) Conception and design: All authors; (II) Administrative support: None; (III) Provision of study material or patients: None; (IV) Collection and assembly of data: All authors; (V) Data analysis and interpretation: All authors; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors.

Correspondence to: He Huang, MD, PhD. Bone Marrow Transplantation Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, No.79 Qingchun Road, Hangzhou 310058, China. Email:

Abstract: Chimeric antigen receptor (CAR) T-cell therapy has achieved great success in recent years, with encouraging complete remission rate and long-term durability of response, especially in advanced B-cell malignancies. With the approval of tisagenlecleucel and axi-cel by FDA to treat refractory/relapsed acute lymphoblastic leukemia and non-Hodgkin lymphoma, our understanding of CAR T cells has been progressing rapidly. In this review, we discussed the designs of CAR T cells, factors affecting response, adverse effects, as well as application beyond B-cell malignancies.

Keywords: Chimeric antigen receptor (CAR); cytokine release syndrome (CRS); neurologic toxicity; acute lymphoblastic leukemia (ALL); non-Hodgkin lymphoma (NHL)

Received: 15 July 2018; Accepted: 11 November 2018; Published: 03 December 2018.

doi: 10.21037/sci.2018.11.05


Chimeric antigen receptor (CAR) T-cell therapy has revolutionized the treatment of relapsed/refractory (r/r) B-cell malignancies. We have seen encouraging complete remission (CR) rate and long-term durability of response in r/r acute lymphoblastic leukemia (ALL) and non-Hodgkin lymphoma (NHL). Acting as a “living drug”, CAR T cells are engineered T cells from patients or previous donors, which can recognize tumor antigens in a major histocompatibility complex (MHC) independent manner.

With the invention of retroviral vectors, T cell engineering began around 1990. The first generation of CAR T cells were developed in 1993 (1) (Figure 1), however, they are not clinically effective due to short persistence. In 1998, the introduction of co-stimulatory domain (2) paved the way for today’s success. In 2003, second-generation CARs (Figure 1) were built to target CD19, which set the stage for the first successful treatment of a patient with ALL in 2011.

Figure 1 Generations of CAR T cells. All generations of CARs have an ectodomain of single chain variable fragment (scFv), an endodomain with signaling modules and domains from CD3ζ, and a transmembrane domain connecting the extracellular and intracellular parts. Different generations differ on the endodomain. For the 1st generation, the endodomain is the CD3ζ. The 2nd generation has co-stimulatory domain and CD3ζ combined, which improved CAR T cell in vivo persistence. The 3rd generation has two consecutive co-stimulatory domains. For the 4th generation, the co-stimulatory domain was combined with the nuclear factor of activated T-cells (NFAT) domain, which are transcription factors that can control cytokine production. CAR, chimeric antigen receptor; DC, dendritic cell.

With the FDA approval of tisagenlecleucel in August 2017 for children and young adults with r/r ALL, and axi-cel in October 2017 for adults with r/r NHL, the research of CAR T-cell therapy has entered a new era. Various new designs and strategies are under development to further boost the efficacy and control the adverse effects of CAR T cells. This mini-review will focus on the current understanding of CAR T cells, discussing the designs, durability of response, adverse effects, as well as its application in other malignant diseases.

Designs of CAR T cells

CARs consist of an ectodomain of single chain variable fragment (scFv) to recognize tumor antigen, an endodomain with signaling modules and domains from CD3ζ, and a spacer and a transmembrane domain connecting the extracellular and intracellular parts. The affinity of scFv influences CAR T-cell function (3); moreover, it could be designed to recognize two or more antigens (tandem CAR or bispecific CAR), rendering CAR T cells to be activated by either or both antigens (4-6). The transmembrane domain which is usually derived from CD3ζ, CD4, CD8, or CD28 molecules, also plays an indispensable role in signal transduction (7). The development of CAR T cells have gone through certain generations (Figure 1), which differs mainly in the endodomain. The first generation of CAR T cells only harbors CD3ζ domain, which lacks persistence. The incorporation of co-stimulatory molecules CD28 and 4-1BB into the intracellular signaling domains significantly promoted CAR T-cell expansion and persistence, leading to today’s breakthrough. CARs harboring two consecutive co-stimulatory domains are called the third-generation CARs. Furthermore, CARs have also been combined with other accessory proteins, such as chemokines (8) and inducible killing switch (9), to improve the function and safety, leading to the so-called fourth generation. Despite the different designs, final activation is initiated by Lck-mediated phosphorylation of CD3 immunoreceptor tyrosine-based activation motifs (10).

The CAR transgene is usually introduced into the genome randomly via γ retroviral transduction, lentiviral transduction, or sleeping beautify system (11). However, random insertion may lead to disruptions of important genes. For example, Fraietta et al. described a case where CAR insertion disrupted TET2 gene (12). Although the accident promoted CAR T-cell proliferation and led to remission, it also carried the risk of oncogenesis. Moreover, the expression of randomly inserted CAR is poorly regulated. Therefore, targeted insertion is preferred. For example, Eyquem et al. used CRISPR/Cas9 system to direct CAR gene to the T-cell receptor α constant (TRAC) locus, which vastly enhanced CAR T-cell potency (13).

Durable response

CAR T-cell therapy was able to achieve high CR rate in heavily pretreated patients with refractory or relapsed diseases. For ALL, Park et al. showed that in 53 patients a CR rate of 83% could be reached (14). For diffuse large B-cell lymphoma (DLBCL), the most recent data from the ZUMA-1 study at the 2018 American Society of Clinical Oncology (ASCO) annual meeting showed that the overall response rate (ORR) at 15.1 months was 79%, with CR rate of 58% (15).

In addition to high CR rate, CAR T-cell therapy has demonstrated durable response, as evidenced by improved survival compared with traditional chemotherapy. For ALL, Park et al. showed that after a median follow-up of 29 months, the median event-free survival (EFS) was 6.1 months, the median overall survival (OS) was 12.9 months (14). For DLBCL, Locke et al. showed that the OS at 18 months was 52% (ZUMA-1) (15). It is becoming clear that patients who maintained CR at month 3 or 6 are likely to experience long-term remission. Some studies have tried to identify factor that could predict the durability of response. For ALL, low disease burden and achievement of CR without minimal residual disease (MRD) after CAR T-cell therapy, but not better CAR T-cell expansion and long-term persistence was associated with better long-term outcome (16). Moreover, Hay et al. reported that patients with normal LDH level prior to lymphodepletion, a platelet count of at least 100 U/L prior to lymphodepletion, and receipt of cyclophosphatidic/fludarabine lymphodepletion regimen were independent predictors of better disease-free survival (DFS) (17). For DLBCL, as reported at the 2018 ASCO meeting, low International Prognostic Index (IPI) score, indolent histology, low lymph node burden, better CAR T-cell expansion and persistence, and higher CD8+ CAR T-cell counts were associated with better outcomes (18). Further studies are needed to confirm these factors in order to better stratify the risks of patients.

One important factor impacting long-term outcome is relapse after CAR T-cell therapy (19). During the 2018 ASCO meeting, Pillai et al. reported that in 150 ALL patients treated with CAR T-cells, 20 had CD19-positive relapses and 33 had CD19 negative relapses (20). Various mechanisms such as CD19 genetic and transcriptional alterations (21-23), lineage switch to myeloid leukemia (22), limited CAR T-cell expansion and persistence (24), and upregulation of immunosuppressive molecules such as PD-1/IDO (11,25) have been identified to cause relapses. Strategies like com bination with PD-1 blockade (26), knockout of CAR T-cell PD-1 gene (27,28), targeting multiple surface markers (5,6) have been implicated.

Adverse effects of CAR T-cell therapy

The two most commonly observed acute adverse effects of CAR T-cell therapy are cytokine release syndrome (CRS) and neurologic toxicity. CRS, the most commonly encountered adverse effect (29), presented as high fever, hypotension, and multi-organ toxicity. It is triggered by activation of CAR T cells and bystander immune cells, upon CAR engagement with antigens expressed by tumor cells. It is characterized by increased levels of various cytokines, including CAR T-cell-derived INF-γ and IL-2, and monocyte-derived IL-6 and IL-1 (30,31) (Figure 2A). Different grading system has been proposed to describe the severity of CRS. The two most commonly used are the UPenn grading scale (32) and the scale proposed by Lee et al. (33) (Table 1). For grade 1 CRS, supportive care alone is usually adequate. For patients with higher grade CRS, more aggressive intervention with combination of anti-IL-6 therapy and/or steroids can usually result in satisfactory control. Recent studies (30,31) using murine models suggested that macrophage played a pivotal role in the CRS; administration of antagonists of macrophage-derived cytokines such as IL-1 and IL-6, or diminishing macrophage function by blocking iNOS, could abate or even prevent the occurrence of CRS.

Figure 2 Models of CRS and neurologic toxicities. (A) CRS is mediated by CAR T cells and bystander immune cells such as monocytes and DCs. In the peripheral blood, when CAR T cells and tumor cells are engaged, CAR T cells are activated and start recruiting monocytes through direct contact such as CD40L-CD40 interaction, or through T-cell derived cytokines such as INF-γ. Activated monocytes can secrete key CRS cytokines IL-1 and IL-6, which in addition to cause symptoms of CRS, can further activate monocytes through iNOS. (B) Neurologic toxicities are caused by cytokines and/or CAR T and non-CAR T cells, secondary to increased BBB permeability. BBB is comprised of ECs and pericytes. Following increased levels of cytokines during CRS, ECs are activated with secretion of angiopoietin 2 (ANG2), which further promotes endothelial activation and microvascular permeability. Next, cytokines such as INF-γ and TNF-α can cross ECs and cause pericyte stress, further increasing BBB permeability. Analysis of CSF showed high levels of IL-2, IL-6, GM-CSF and VEGF, as well as CAR T cells and non-CAR T cells. CRS, cytokine release syndrome; CAR, chimeric antigen receptor; DC, dendritic cell; iNOS, inducible nitric oxide synthase; BBB, blood-brain barrier; EC, endothelial cells; CSF, cerebrospinal fluid.
Table 1
Table 1 Common grading systems for cytokine release syndrome and neurologic toxicity
Full table

Neurologic toxicity, typically presented as confusion, delirium, seizure and cerebral edema, is the second most common acute adverse effect. It can occur concurrently with CRS or have a delayed onset even weeks after infusion. The mechanism of neurologic toxicity still remains unclear; it could be caused by passive diffusion of cytokines into the brain (35) or trafficking of CAR T cells into the central nervous system (CNS) (36). A recent study utilized a rhesus macaque model showed that neurologic toxicity was associated with increased levels of pro-inflammatory cytokines and pan-T cell infiltration (both CAR T cells and non-CAR T cells) (37) (Figure 2B). Pathology of patients with severe neurologic toxicity revealed endothelial activation, with capillary leak and increased blood-brain barrier (BBB) permeability (38). Neurologic toxicity can be graded based on the Common Terminology Criteria for Adverse Events (CTCAE) criteria or the grading system proposed by Neelapu et al. (29) (Table 1). For the management of neurologic toxicity, anti-IL-6 tocilizumab is believed ineffective without concurrent CRS, because this antibody is unable to cross BBB. The effectiveness of IL-1 receptor blocker in neurologic toxicity is still under examination (31).

CAR T-cell therapy can also cause other adverse effects, such as cytopenia, on-target/off-tumor recognition, and graft-versus-host disease if CAR T cells are allogeneic (39). At last, it is worth noting that toxicity varies in different CAR T-cell designs and in different disease types (40).

CAR T-cell therapy beyond B-cell malignancies

Although CAR T-cell therapy is currently approved to treat only relapsed/refractory ALL and NHL, evidence of its effectiveness in other malignancies is emerging. For example, B-cell mature antigen (BCMA)-targeting CAR T cells have shown promising results in patients with multiple myeloma (MM). At the 2017 ASCO meeting, a single armed clinical trial reported 18 (95%) of 19 patients achieved CR or near CR throughout a median follow-up of 208 days (41). In another report, Brudno et al. (42) showed an ORR of 81%, with 63% CR or near CR. Despite the promising results, one of the biggest concerns of BCMA-targeting CAR T cells is that BCMA can be cleaved from cell surface and shed into blood, resulting in antigen loss. Indeed, BCMA loss has been reported in some studies (42,43).

For acute myeloid leukemia, researchers are yet able to replicate the success in ALL and MM. The biggest challenge we are facing is to identify the optimal myeloid antigen, without causing significant on target/off tumor toxicity. Various targets, such as CD33, CD123, NKG2DL were currently examined in phase I clinical trials (44).

The success of CAR T cells in hematologic malignancies has yet to be extrapolated to solid tumors due to the lack of specific targetable antigens, on-target/off-tumor effect and the complex tumor microenvironment (45). Indeed, significant solid tumor regression without severe toxicities has yet to be reported except in one case where glioblastoma was successfully treated with intracranial IL13Rα2-targeting CAR T cells (46). Different strategies, such as regional delivery of CAR T cells (47,48), design of combinational-targeting CARs (49), engineering CAR T cells to directly deliver cytokines within the tumor (50), combination with checkpoint inhibitors (51), and combination with oncolytic virus therapy (52,53), have shown promising results in pre-clinical studies.

Future perspectives

With the approval of CAR T-cell therapy in advanced B-cell malignancies, our understanding of CAR T-cell therapy has been progressing rapidly. With longer follow-ups and more patients receiving CAR T-cell therapy, we would be able to stratify the risks in patients and predict the clinical outcomes. New therapeutic targets in the CRS and neurologic toxicities, such as IL-1 and iNOS, have been identified in pre-clinical studies; their roles should be confirmed in future research. Anyhow, we are walking toward more efficient and safer CAR T cells. At last, the success of CAR T-cell therapy in hematological malignancies has yet to be replicated in solid tumors. It requires great effort to tackle the unique challenges in solid tumors.


Funding: This work was supported by the grants from 973 Program (grant No. 2015CB964900), the Natural Science Foundation of China (grant No. 81230014, 81470341, 81520108002, 81500157), Key Project of Science and Technology Department of Zhejiang Province (grant No. 2015C03G2010091).


Conflicts of Interest: The authors have no conflicts of interest to declare.


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doi: 10.21037/sci.2018.11.05
Cite this article as: Wang J, Hu Y, Huang H. Current development of chimeric antigen receptor T-cell therapy. Stem Cell Investig 2018;5:44.