Leptomeninges: a novel stem cell niche with neurogenic potential
Editorial

Leptomeninges: a novel stem cell niche with neurogenic potential

Takayuki Nakagomi, Tomohiro Matsuyama

Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo, Japan

Correspondence to: Takayuki Nakagomi. Institute for Advanced Medical Sciences, Hyogo College of Medicine, 1-1 Mukogawacho, Nishinomiya, Hyogo, 663-8501, Japan. Email: nakagomi@hyo-med.ac.jp.

Provenance: This is an invited Editorial commissioned by Editor-in-Chief Zhizhuang Joe Zhao (Pathology Graduate Program, University of Oklahoma Health Sciences Center, Oklahoma City, USA).

Comment on: Bifari F, Decimo I, Pino A, et al. Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex. Cell Stem Cell 2017;20:360-373.e7.


Received: 01 March 2017; Accepted: 19 March 2017; Published: 31 March 2017.

doi: 10.21037/sci.2017.03.09


The meninges are composed of three membranous layers referred to as the dura mater, arachnoid mater, and pia mater. The arachnoid mater and pia mater are connected together and form the leptomeninges. The meninges not only protect the central nervous system (CNS), including the brain and spinal cord, directly with their thick outer layers but also indirectly by cushioning through the cerebrospinal fluid filled in the subarachnoid space. In addition, a previous study demonstrated that removing the meninges causes impaired cortical development (1). This suggests that the meninges have functions other than protection. In support of this idea, it is reported that the meninges modulate cortical neuron generation during early brain development by producing trophic factors such as retinoic acid (2). Thus, it is likely that they play pivotal roles in cortical neurogenesis by regulating embryonic neural stem/progenitor cells (NSPCs) (3).

In addition to these roles and effects, increasing evidence now shows that the meninges themselves function as a stem cell niche. Bifari et al. first reported that the leptomeninges in developing mouse brain express the NSPC marker nestin and that nestin+ cells isolated from the leptomeninges exhibited activities of NSPCs, which differentiate into neurons in vivo and in vitro (4). We later showed that the leptomeninges isolated from intact brain regions in adult mice lack stem cell potential (5). These findings showed that leptomeningeal NSPCs have potential to contribute to cortical neurogenesis during early developmental stages.

Using adult mice following ischemic stroke, we previously showed that nestin+ ischemia-induced NSPCs (iNSPCs) were present in the leptomeninges of ischemic areas, and they were not observed in the leptomeninges of nonischemic areas (5). Leptomeningeal cells isolated from ischemic areas produce neurosphere-like cell clusters that give rise to neural cells, including neurons (5). Furthermore, we showed that labeled leptomeningeal cells from ischemic areas migrated into post-stroke areas of the cortex and that they differentiated into doublecortin (DCX)+ immature neuronal cells (6). These findings indicate that under pathological conditions, such as after ischemic stroke, leptomeningeal iNSPCs can contribute to brain repair through cortical neurogenesis.

The leptomeninges are histologically continuous with the cortical parenchyma along the blood vessels and are located at a perivascular niche as vascular pericytes (7). We showed that leptomeningeal nestin+ cells spread into the cortical parenchyma, localized near CD31+ endothelial cells, and expressed pericytic makers such as PDGFRβ and NG2 (5). Although the functions of pericytes remain unclear, it has been well-documented that pericytes in various organs, including the CNS, have multipotent stem cell activity (8). However, using mice at different developmental stages, including embryonic, postnatal, and adult, we recently showed that brain pericytes gradually decreased their stemness during the postnatal period and lost it by adulthood (9). Therefore, it is likely that adult brain pericytes have the features of somatic cells rather than those of tissue-committed stem cells. However, reprogramming has been reported to cause adult brain pericytes to become non-pericytic lineages, such as neuronal lineages (10). In support of this notion, we showed that adult brain pericytes, which do not possess stem cell activity under normal conditions, reacquired stemness in response to ischemia, presumably through cellular reprogramming by mesenchymal–epithelial transition (11,12). We also showed that PDGFRβ+ cells isolated from ischemic areas, including the leptomeninges, have a multipotent stem cell activity that gives rise to neuronal cells (11,12). Therefore, we proposed that brain pericytes localized along the leptomeninges to cortical parenchyma are likely the origin of leptomeningeal stem cells.

Very recently, Bifari et al. reported that the leptomeninges in the neonatal brain harbor radial glia-like cells resembling NSPCs in the subventricular zone (13). In addition, they showed that leptomeningeal radial glia-like neural progenitors migrate from the leptomeninges to the cortex and differentiate into functionally integrated cortical neurons. These findings were consistent with those of our previous report that showed that cortical neurons originate in part from leptomeningeal NSPCs (6). However, leptomeningeal radial glia-like neural progenitors are likely neuronal progenitors rather than NSPCs because they differentiated into neuronal lineages expressing HuC/D, DCX, NeuN, and Stab2, but not into astrocyte and oligodendrocyte lineages. Furthermore, using genetic mapping with PDGFRβ through Cre-loxP system, a technique to selectively label leptomeningeal cells, and single-cell transcriptomics, Bifari et al. concluded that cortical neurons are in part derived from PDGFRβ+ radial glia-like neural progenitors in the leptomeninges (13). Of note, using single-cell transcriptomics, they also showed that leptomeningeal PDGFRβ+ cells generate various types of clusters that exhibited features of pericytic/fibroblastic, endothelial, and microglial lineages in addition to radial glia-like lineages. However, we recently showed that PDGFRβ+ pericytes isolated from post-stroke areas, including the leptomeninges, exhibited multipotent stem cell activity and that they give rise to not only neural (e.g., neurons) but also vascular lineages (e.g., endothelial cells and microglia) (11,12). Thus, it is possible that such various phenotypes exhibited by PDGFRβ+ leptomeningeal cells are due to their original multipotency. Previously, we also showed that nestin+/NG2+/PDGFRβ+ iNSPCs from ischemic areas, including the leptomeninges, which likely originated from brain multipotent pericytes, expressed a mesenchymal marker vimentin (5,6). However, vimentin is also expressed in radial glia-like cells (14). Although the relationships between pericytes, glia, and NSPCs remain unclear (15), Birbrair et al. divided multipotent pericytes into two subtypes (type-1 and type-2 pericytes). They further demonstrated that nestin+/NG2+/PDGFRβ+ type-2 pericytes have the potential to differentiate into neural lineages (16). Of interest, type-2 pericytes have traits resembling neural progenitors that exhibit properties of NG2-glia (17). Therefore, it is possible that we merely look at subsets of the same PDGFRβ+ leptomeningeal cells during different stages (e.g., neonatal vs. adult) and/or under different conditions (e.g., normal vs. pathological).

The precise traits of PDGFRβ+ leptomeningeal cells should be clarified in further investigations. However, accumulating evidence shows that the leptomeninges that cover the entire CNS, including the brain (4-6,13,18,19) and spinal cord (20), harbor stem cell-like populations that differentiate into neuronal cells. The leptomeningeal stem/progenitor cells were observed not only during early development under normal conditions (4,13,18) but also during adulthood under pathological conditions (5,6,19,20). Thus, the leptomeninges should become a new target for treating CNS developmental disorders and diseases.


Acknowledgements

The authors wish to thank members of the laboratory for helpful assistance.


Footnote

Conflicts of Interest: The authors have no conflicts of interest to declare.


References

  1. Halfter W, Dong S, Yip YP, et al. A critical function of the pial basement membrane in cortical histogenesis. J Neurosci 2002;22:6029-40. [PubMed]
  2. Siegenthaler JA, Ashique AM, Zarbalis K, et al. Retinoic acid from the meninges regulates cortical neuron generation. Cell 2009;139:597-609. [Crossref] [PubMed]
  3. Sockanathan S, Gaiano N. Preview. Meninges Play a RAdical Role in Embryonic Neural Stem Cell Regulation. Cell Stem Cell 2009;5:455-6. [Crossref] [PubMed]
  4. Bifari F, Decimo I, Chiamulera C, et al. Novel stem/progenitor cells with neuronal differentiation potential reside in the leptomeningeal niche. J Cell Mol Med 2009;13:3195-208. [Crossref] [PubMed]
  5. Nakagomi T, Molnar Z, Nakano-Doi A, et al. Ischemia-induced neural stem/progenitor cells in the pia mater following cortical infarction. Stem Cells Dev 2011;20:2037-51. [Crossref] [PubMed]
  6. Nakagomi T, Molnar Z, Taguchi A, et al. Leptomeningeal-derived doublecortin-expressing cells in poststroke brain. Stem Cells Dev 2012;21:2350-4. [Crossref] [PubMed]
  7. Hutchings M, Weller RO. Anatomical relationships of the pia mater to cerebral blood vessels in man. J Neurosurg 1986;65:316-25. [Crossref] [PubMed]
  8. Dore-Duffy P, Katychev A, Wang X, et al. CNS microvascular pericytes exhibit multipotential stem cell activity. J Cereb Blood Flow Metab 2006;26:613-24. [Crossref] [PubMed]
  9. Nakano-Doi A, Nakagomi T, Sakuma R, et al. Expression patterns and phenotypic changes regarding stemness in brain pericytes in health and disease. J Stem Cell Res Ther 2016;6:332. [Crossref]
  10. Karow M, Sanchez R, Schichor C, et al. Reprogramming of pericyte-derived cells of the adult human brain into induced neuronal cells. Cell Stem Cell 2012;11:471-6. [Crossref] [PubMed]
  11. Nakagomi T, Kubo S, Nakano-Doi A, et al. Brain vascular pericytes following ischemia have multipotential stem cell activity to differntiate into neural and vascular lineage cells. Stem Cells 2015;33:1962-74. [Crossref] [PubMed]
  12. Sakuma R, Kawahara M, Nakano-Doi A, et al. Brain pericytes serve as microglia-generating multipotent vascular stem cells following ischemic stroke. J Neuroinflammation 2016;13:57. [Crossref] [PubMed]
  13. Bifari F, Decimo I, Pino A, et al. Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex. Cell Stem Cell 2017;20:360-73.e7. [Crossref] [PubMed]
  14. Sundholm-Peters NL, Yang HK, Goings GE, et al. Radial glia-like cells at the base of the lateral ventricles in adult mice. J Neurocytol 2004;33:153-64. [Crossref] [PubMed]
  15. Nakagomi T, Nakano-Doi A, Kawamura M, et al. Do Vascular Pericytes Contribute to Neurovasculogenesis in the Central Nervous System as Multipotent Vascular Stem Cells? Stem Cells Dev 2015;24:1730-9. [Crossref] [PubMed]
  16. Birbrair A, Zhang T, Wang ZM, et al. Skeletal muscle pericyte subtypes differ in their differentiation potential. Stem Cell Res 2013;10:67-84. [Crossref] [PubMed]
  17. Birbrair A, Zhang T, Wang ZM, et al. Skeletal muscle neural progenitor cells exhibit properties of NG2-glia. Exp Cell Res 2013;319:45-63. [Crossref] [PubMed]
  18. Bifari F, Berton V, Pino A, et al. Meninges harbor cells expressing neural precursor markers during development and adulthood. Front Cell Neurosci 2015;9:383. [Crossref] [PubMed]
  19. Ninomiya S, Esumi S, Ohta K, et al. Amygdala kindling induces nestin expression in the leptomeninges of the neocortex. Neurosci Res 2013;75:121-9. [Crossref] [PubMed]
  20. Decimo I, Bifari F, Rodriguez FJ, et al. Nestin- and doublecortin-positive cells reside in adult spinal cord meninges and participate in injury-induced parenchymal reaction. Stem Cells 2011;29:2062-76. [Crossref] [PubMed]
doi: 10.21037/sci.2017.03.09
Cite this article as: Nakagomi T, Matsuyama T. Leptomeninges: a novel stem cell niche with neurogenic potential. Stem Cell Investig 2017;4:22.

Refbacks

  • There are currently no refbacks.